The Antibody Stains CD3+ Cells Very Well

July 22, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse CD3ε Antibody

Catalog Number:

100336

We wanted to study the T-cells in the intestine in a diseased vs healthy state, so we used this antibody to identify T-cells by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of colon cells

Primary Incubation

1:100

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody distinguishes CD3+ cells very clearly

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Antibody stains CD3+ T-cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying T-cells

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