Workhorse Instrument

University of South Alabama
Basic Medical Sciences
Graduate Student

Overall

Performance

Ease-of-Optimization

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Company:

ThermoFisher Scientific

Product Name:

LTQ Orbitrap XL

Catalog Number:

IQLAAEGAAPFADBMAOK

We do bottom-up proteomics research using this instrument. We often run this instrument 24 hours a day for multiple consecutive days with few, if any, issues.

Experimental Design and Results Summary

Application

Proteomics

Starting Material

Biofluids, primarily serum

Protocol Overview

Proteins isolated from biofluids using solid phase extraction. Trypsin digestion and LC-MS/MS.

Tips

Stay away from detergents

Results Summary

Very useful instrument as the machine is able to acquire MS and MS/MS data simultaneously. No loss of data while machine is analyzing fragment data. The machine has a linear ion trap for lower resolution spectrum and an Orbitrap for high resolution analysis.

Features Summary

Hybrid instrument for acquiring MS and MS/MS data simultaneously

Additional Notes

None

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Summary

The Good

Hybrid instrument for continuous data acquisition

The Bad

Lacks the sensitivity of the newer instruments

The Bottom Line

Quality instrument from a trusted manufacturer. Solid performance over a period of years.

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