Good Antibody To Detect Murine MHC-II Expression

July 14, 2016

Biomedical Sciences
TTUHSC
Lead Technician

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse MHC Class II (I-A/I-E) eFluor® 450

Catalog Number:

48-5321-82

I used this antibody to detect MHC-II expression in mouse splenocytes, BMMs, and BMDCs.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Bone marrow derived Dendritic cells

Primary Incubation

30 mins

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry in PB Channel

Results Summary

I was able to detect MHC-II expression on BMDCs using this antibody. The result confirm the usefulness of this antibody in detecting mouse MHC-II expression.

Additional Notes

None

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Summary

The Good

Reproducibility

The Bad

None

The Bottom Line

Excellent antibody to detect mouse MHC-II expression

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