Good Separation Using This Antibody

July 22, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC Anti-mouse CD4

Catalog Number:

100406

This antibody was used to gate on the CD4 T cells in a mixed population after in vitro culture.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse lymphocytes

Primary Incubation

0.25 µg per million cells in 100 µl volume (manufacturer's recommendation) 30minutes on ice.

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Fortessa

Results Summary

The positive peak is bright enough to gate on.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright

The Bad

None

The Bottom Line

Reliable antibody for experiments.

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