Not a Good Fluorochrome for CD4 Detection

June 30, 2016

Immunology
University of Rochester
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Bioscience

Product Name:

BV786 Rat Anti-Mouse CD4

Catalog Number:

563727

Aim of using this product is to determine how pure the isolated CD4 T cell sample was after cell separation.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Mouse splenocytes, mouse CD4 T cells

Primary Incubation

1:200 in FACS buffer. Then the cells were stained with a live/dead marker.

Blocking Agent

none

Secondary Incubation

none

Tertiary Incubation

none

Detection

Fortessa

Results Summary

The positive population is not bright enough to gate on. Other fluorochromes gave a better separation on the histogram.

Additional Notes

None

Related Categories

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Summary

The Good

Cost

The Bad

Staining is not bright enough

The Bottom Line

BV786 is not a good fluorochrome for staining brightly.

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