Excellent Kit for Isolating Untouched Human Memory CD4+ T cells

TTUHSC El Paso
Dept of biomedical Sciences
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

STEMCELL Technologies

Product Name:

EasySep™ Human Memory CD4+ T Cell Enrichment Kit

Catalog Number:

19157

This kit was used for isolating untouched memory CD4 T cells from human PBMCs.

Experimental Design and Results Summary

Application

Magnetic cell isolation

Starting Material

Human PBMcs

Protocol Overview

1. Resuspend cells in the suggested medium (check the data sheet) 2. incubate with EasySep™ Human memory CD4 T cell enrichment Cocktail ( as per data sheet) for 10 minutes 3. Incubate with D magnetic particles for 5 minutes at RT4. Magnetic separation for 2.5 minutes 5. Check the purity of the negatively isolated cells

Tips

Follow the protocol in data sheet

Results Summary

Isolated a pure untouched CD4 memory T cell population

Additional Notes

None

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Summary

The Good

Good kit for isolating CD4 memory T cells

The Bad

None

The Bottom Line

Excellent kit for isolting CD4 memory T cells

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