Excellent Kit for Isolating Untouched Human CD4 T Cells

TTUHSC El Paso
Dept of biomedical Sciences
Post doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

STEMCELL Technologies

Product Name:

EasySep™ Human CD4+ T Cell Enrichment Kit

Catalog Number:

19052

This kit can be used for isolating untouched human CD4 T cells from whole PBMCs.

Experimental Design and Results Summary

Application

Magnetic cell isolation

Starting Material

Human PBMcs

Protocol Overview

1. Resuspend cells in the suggested medium (check the data sheet) 2. incubate with EasySep™ T cell enrichment Cocktail ( as per data sheet) for 10 minutes 3. Incubate with D magnetic particles for 10 minutes at RT4. Magnetic separation for 5 minutes 5. Check the purity of the negatively isolated cells

Tips

Follow the protocol in data sheet

Results Summary

Was able to isolate pure population of human CD4 positive cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Easy protocol

The Bad

None

The Bottom Line

Good kit for isolating untouched CD4 T cells.

Join the discussion