Fast Kit to Determine IL-6 Levels

University of California
Internal Medicine
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Overall

Quality of Results

Ease-of-Optimization

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Company:

EBioscience

Product Name:

Mouse IL-6 ELISA Ready-SET Go

Catalog Number:

88-7064-22

The aim of the study was determine the levels of IL-6 in two cohorts of mice injected with two compounds A & B. Due to undetermined levels previously we aimed for a ready made kit without the need for optimization.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Serum samples from mice injected with molecules A & B

Protocol Overview

EBioscience ready-SET go kit came with instructions to follow that were used as per instructions provided

Tips

Use only the substrate from the kit. The substitution of substrate results in variable results

Results Summary

Sensitivity is good enough to detect as low as 15pg/ml

Additional Notes

None

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Summary

The Good

Fast and sensitive enough for assay

The Bad

Substrate problems if switched to any other company

The Bottom Line

Overall good product and is also not so expensive

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