Biolegend
PerCP anti-human CD4 Antibody
317431
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Browse Similar Products: CD4 Flow Cytometry Antibodies
In order to discriminate CD4+ T cells from other PBMC, we labeled the cells with the above antibody. Further applications included the characterization of Th1 Th2 cells and regulatory T cells.
Flow Cytometry
Primary Human PBMC
5µl per 1 million cells, incubation 20 Mins on ice in the dark, was 3 times with FACS buffer (PBS, 1% BSA, 0,001% Na Azide)
Tru Stain FcX Receptor blocking solution (Biolegend)
None
Flow Cytometry, Attune Acoustic Focusing Cytometer
This antibody clearly discriminated CD4+ from CD4- cells in order to separate the cells for further downstream panels such as Th1 Th2 or to Treg identification.
Be sure to Fc Block prior toadding the antibody. When using ledd than 1 million cells you may also reduce the amount of antibody.
Easy to use, recommended titration works.
Washing 3 times
Really easy to use. Antibody produces reproducible results, robust against photobleaching, very specific, low background.