Invitrogen/Life Technologies/Thermo
anti-GPX1 antibody (anti-glutathione peroxidase 1)
PA5-26323
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We wanted to detect glutathione peroxidase 1 (GPX1) in HK2 cells (human kidney cell line). We selected this antibody because it was the least expensive antibody for this antigen from any of the companies that we trust (we don't trust all companies!). We aliquotted this antibody and stored it as 10uL aliquots in our -80 freezer. We actually made 48 aliquots from it, and we only expected 40, so Invitrogen was generous.
Western Blot
HK2 cells
1:1000, overnight, 4 deg C, rocking
Odyssey Blocking Buffer
1:20,000, 1 hr, room temp
None
LiCor Odyssey Scan
Secondary antibody was 925-68073 from LiCor, donkey anti-Rabbit 680 nm.The blot was clean except for some faint non-specific bands just above the GPX band. But the GPX band was so strong that the faint bands did not matter much.
It worked great, the price was fine, there was more in the vial than expected.
Some faint bands at too-high molecular weight may or may not be specific
I recommend this antibody for Western blotting.