Strong CD45 Staining

Immunology
University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse CD45 PE

Catalog Number:

12-0451

We used CD45 to identify hematopoietic cells from lymph nodes or spleens. Our research investigates antigen processing and presentation pathways in different populations of murine cells.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:1000 for 20 minutes at 4 degC

Blocking Agent

2.4G2 Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Canto II flow cytometer

Results Summary

CD45 staining is always strong and consistent. It works well on all colors tested.

Additional Notes

None

Image Gallery

Summary

The Good

Strong consistent staining

The Bad

None

The Bottom Line

This antibody is a great way to identify hematopoietically-derived cells.

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