eBioscience
Anti-Mouse CD31 (PECAM-1) PerCP-eFluor® 710
46-0311
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Browse Similar Products: CD31 Flow Cytometry Antibodies
We used this antibody to identify endothelial cells, which are CD45 negative and CD31 positive. Our research investigates the ability of endothelial cells from tissues or lymph nodes to modulate immune responses.
Flow Cytometry
Murine lymph nodes
1:1000 for 20 minutes at 4 degC
2.4G2 Fc block
None
Canto II flow cytometer
CD31 gives strong endothelial cell staining, which works well on most colors we have tested. In applications where endothelial cells are rare, it is important to also use CD45 to exclude hematopoietic cells, and to use a live/dead marker to exclude dead cells.
Strong consistent CD31 staining
Good CD31 staining