Consistent CD11b staining

Immunology
University
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Mouse CD11b (Mac-1) PerCPCy5.5 clone M1/70

Catalog Number:

45-0112

We used this antibody to identify CD11b+ cells to help distinguish macrophages, dendritic cells, and other populations of APC. Our research investigates antigen processing and presentation pathways in different populations of murine lymphocytes.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Murine lymphocytes

Primary Incubation

1:500 for 20 minutes at 4 degC

Blocking Agent

2.4G2 Fc block

Secondary Incubation

None

Tertiary Incubation

None

Detection

Canto II flow cytometer

Results Summary

This antibody consistently and reproducibly stains CD11b+ cells. It works well on a variety of colors tested.

Additional Notes

None

Image Gallery

Summary

The Good

Reliable staining

The Bad

None

The Bottom Line

Consistent reliable staining on multiple different colors tested.

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