PE Mouse IgG2b kappa Isotype Control Flow Cytometry Staining

June 30, 2016

Inflammation & Ageing
University of Birmingham
PhD Student

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Company:

BD Biosciences

Product Name:

PE Mouse IgG2b κ Isotype Control Clone 27-35 (RUO)

Catalog Number:

555743

Browse Similar Products: IgG2b kappa Flow Cytometry Antibodies

The isotype control was used to stain human monocytes isolated from peripheral blood to control for non-specific/Fc receptor binding of the antibody isotype

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human monocytes isolated from leukapheresis products.

Primary Incubation

30 minute incubation at 4oC. Antibody was used at a 1 in 100 diltuion

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR X-20 Fortessa. Analysis using FlowJo v10

Results Summary

The antibody performed well as an isotype control with little to no non-specific binding to human peripheral blood monocytes

Additional Notes

None

Image Gallery

Summary

The Good

Antibody performed as expected allowing quantification of non-specific binding

The Bad

No particular bad points

The Bottom Line

The antibody worked as one would wish with little non-specific binding to human monocytes, a good isotype control.

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