BD Biosciences
FITC Mouse IgG1, κ Isotype Control Clone MOPC-21 (RUO)
555909
Browse Similar Products: IgG1 Flow Cytometry Antibodies
This antibody was used to control for non-specific/Fc receptor binding on human monocytes isolated from peripheral blood.
Flow Cytometry
Human monocytes isolated from leukapheresis products.
30 minute incubation at 4oC. Antibody was used at a 1 in 10 diltuion
No specific blocking step. 2% FCS in staining buffer.
None
BD LSR X-20 Fortessa. Analysis using FlowJo v10
The antibody worked well with very little positive staining providing a decent control for non-specific/Fc receptor binding
Antibody worked as expected with little background
No particular bad points
A decent isotype control that works as one would want