Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody Flow Cytometry Staining Using PE-Cy7 Streptavidin

June 27, 2016

Inflammation & Ageing
University of Birmingham
PhD Student

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

R & D Systems

Product Name:

Human Fibroblast Activation Protein alpha /FAP Biotinylated Antibody

Catalog Number:

BAF3715

We used the antibody in combination with PECy7 conjugated streptavidin to detect FAP expression on human synovial cells yielded by enzymatic digestion.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human synovial cells

Primary Incubation

30 minute incubation at 4oC. Live/dead stain.

Blocking Agent

No specific blocking step. 2% FCS in staining buffer.

Secondary Incubation

30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 2ug/ml.

Tertiary Incubation

30 minute incubation at 4oC with PE-Cy7 streptavidin conjugate at 1 in 250 dilution

Detection

Mo Flow Astrios EQ 561 excitation 795/70 bandpass filter for emission

Results Summary

Bright staining allowed clear identification of FAP positive cells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Nice bright antibody supplied with a reasonable amount.

The Bad

No particular bad points

The Bottom Line

A decent antibody that works as expected

Share your experience with other scientists. Write a Review! »

Join the discussion