Epithelial Progenitor Marker

Department of Urology
UT Southwestern Medical Center
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Miltenyi Biotec

Product Name:

CD133/1 PE/Vio770

Catalog Number:

130-101-652

Flow cytometry was used to probe epithelial dynamics and the immune infiltrates in human prostate tissues. Epithelia was investigated with CD45-CD325+ cells. Leukocytes were looked at using CD326-CD45+. Sub-populations (such as epithelial stem cells or individual leukocyte populations like T-Cells) were gated with appropriate markers.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Primary human prostate cells

Primary Incubation

Recommended for 20-30mins

Blocking Agent

FcX

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometer

Results Summary

Epithelial Stem cells +ve

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

OK Brightness

The Bad

Not good separation between positives and negatives

The Bottom Line

Good especially if used with other progenitor markers

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