Good Human IL-17 A Antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-human IL-17A Antibody

Catalog Number:

512304

Human PBMC were stimulated for 6 hrs with PMA/Ionomycin in the presence of GolgiStop. Cells were surface stained first with Pacific blue conjugated anti-human CD4 antibody. Surface stained cells were washed and later fixed and permeabilized using Cytofix/Cytoperm reagents followed by staining with FITC anti-human IL-17A . The cells were finally analyzed by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry in FITC and PB Channel

Results Summary

Presence of IL-17A positive cells confim the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Specificity

The Bad

None

The Bottom Line

Good antibody for detecting human IL-17 A expression

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