Good Anti-Human CD68 Antibody

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

FITC anti-human CD68 Antibody

Catalog Number:

333806

Human monocytes were fixed and permeabilized using cytoperm/cytofix kit. These fixed and permeabilized celsl were intra-cellularly stained with either isotype of FITC conjugated CD68 antibody. Cells were washed after 30 min and analyzed by flow cytometry

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry on FITC Channel

Results Summary

Was able to detect CD68 expression which confirm the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Specificity

The Bad

Intra-cellular staining is required for detection

The Bottom Line

Good antibody to detect CD68 expression in human cells

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