5 PRIME, USA
HotMaster Mix Taq DNA polymerase
2200310
DNA was purified and DNA template was amplified by using HotMaster Mix Taq DNA polymerase using specific primers for PEPCK (phosphoenol pyruvate carboxykinase) for fast PCR. Amplified product was run in agarose gel.
Amplification of highly specific or complementary DNA template and detects low low copy number genes.
DNA isolated from liver cells
DNA was isolated from rat liver cells. DNA template was then amplified by using highly specific primers. The reaction mixture contains DNA template, primers, HotMaster Mix Taq DNA polymerase, HotMaster TaqBuffer and dNTPs. The PCR tube containing the reaction mixture was then kept in PCR machine and amplified by setting up of thermocycling conditions. Here no separate step for activation of DNA polymerase is required.
Tips: It should be stored at -20°C
The amplified DNA product was run on the agarose gel and very sharp band was observed which indicated no non-specific amplification.
None
The reaction mixture can be set up on bench and activation of DNA polymerase is not required. Up to 5 kb of DNA fragment can be amplified.
Fast DNA amplification by HotMaster Mix Taq DNA polymerase