Enzo Life Sciences
Anti-KDEL
ADI-SPA-827-J
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Browse Similar Products: KDEL Western Blot Antibodies
To asses ER-stress, astrocytes were treated 24hs with tunicamycin as a positive control of ER-stress. Total proteins were analyzed by western blot against KDEL, to quantify chaperone's over-expression
Western Blot
Protein extract of cultured astrocytes
1:2000, Over night, 4˚C, incubated in 5% slime milk in TBS-T
5% slime milk in TBS-T
Goat anti-Rabbit IgG-HRP 1:4000, 1 hour, room temperature, incubated in 5% slime milk in TBS-T
Not necessary
Chemiluminescent detection
Western blot showed 3 bands at different weights: 94-95KDa (GRP94), 78KDa (GRP78) and approximately a 55KDa protein (probably Calreticulin). Western blot showed increase expression of chaperones in positive tunicamycin treated cells.
None
Without any background
Nothing
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