GE Healthcare
Anti-BrdU Antibody
RPN202
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To analyze proliferation, 70% confluent culture of astrocytes were labeled with 5uM BrdU and revealed with anti-BrdU antibody.
Immunofluorescence
Rat astrocyte purified culture
1:100, 45 minutes at room temperature
2% BSA
1:500, 90 minutes, Room Temperature, in PBS
Counter-staining for 5 minutes with 1ug/ml DAPI
Fluorescence (FITC)
The antibody works really good,
Cells were fixed with ice-cold Methanol, and treated 15 minutes with 2N HCl
Excellent result
Nothing
Good and reliable result in a short period of time