Applied Biosystems, life technologies, UK
SYBR Green PCR Master Mix
4309155
Gene expression of G6Pase (Glucose 6 Phospatase) was studied in liver samples. Total RNA was isolated. RNA is then converted into cDNA which is amplified by SYBR Green PCR Master Mix by following the kit protocol.
Gene expression analysis
Rat liver sample
Rat liver was homogenized in TRIZOL reagent and total RNA was isolated by TRIZOL method. The isolated RNA was then converted into cDNA. cDNA template was amplified by adding primers and SYBR Green PCR Master Mix according to the kit instructions and amplified in Real-Time PCR machine. Contents were mixed properly. Amplification parameters were also followed from the kit. The SYBR Green dye binds with the double stranded DNA during amplification and fluorescence was detected.
Kit should be stored at 2 to 8°C
The amplification was done Real-Time PCR machine and amplified products were observed in 3% agarose gel.
None
This kit save time and it is simple to perform
Gene expression analysis by SYBR Green PCR Master Mix