IHC Analysis

Pharmacology
University of Colorado
Postdoc

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Quality of Results

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Company:

Cell Signaling

Product Name:

VEGF R2 (55B11)

Catalog Number:

2479S

This antibody was used to analyze VEGFR2 protein expression in prostate cancer xenograft tissue. This antibody gave best results at 1:150 dilution in PBS for overnight incubation. This antibody was further detected by biotin labeled linked secondary antibody at dilution of 1:200 for 1 hour.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Prostate xenograft tissue

Primary Incubation

1:150

Blocking Agent

CAS block

Secondary Incubation

1:200

Tertiary Incubation

1:500

Detection

HRP detection system from VECTOR Labs

Results Summary

This antibody shows excellent VEGFR2 protein localization in prostate cancer xenograft tissue.

Additional Notes

None

Related Categories

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Summary

The Good

Specificity

The Bad

Nothing

The Bottom Line

This antibody is good for IHC and WB.

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