Cell Signaling LC3B Antibody Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

LC3B (D11) XP® Rabbit mAb

Catalog Number:

3868S

We used this antibody to look at autophagy occurrence and inhibition in mouse ES cells after different treatments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% BSA

Secondary Incubation

1:3000 in 5% BSA for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, clear expected bands.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Clear expected band

The Bad

N/A

The Bottom Line

Great autophagy marker, would use again!

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