Cell Signaling LC3A Antibody Review

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

LC3A (D50G8) XP® Rabbit mAb

Catalog Number:

4599S

We used this antibody to measure autophagy/inhibition of autophagy after giving mouse ES cells different treatments. We chose this antibody because we've heard great things about it from other labs.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% BSA

Secondary Incubation

1:3000 in 5% BSA for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

This is a great antibody. Strong clear bands for lc3a I and II. No background.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Nice clear specific bands

The Bad

N/A

The Bottom Line

Great antibody! Would use again!

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