PAX8 Antibody Review

May 31, 2016

Center for Regenerative Medicine
Boston University
Postdoctoral Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Proteintech

Product Name:

PAX8 Rabbit PolyAb

Catalog Number:

10336-1-AP

The aim of this study was to detect Pax8 in mouse thyroid sections.

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Mouse thyroid sections

Primary Incubation

1:100

Blocking Agent

4% Donkey Serum

Secondary Incubation

Invitrogen donkey-anti-rabbit

Tertiary Incubation

Not applicable

Detection

Fluorescent Microscopy

Results Summary

It was easy to detect the PAX8 positive-cells in the tissue sections.

Additional Notes

Use a low PH antigen retrieval step.

Related Categories

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Summary

The Good

Good customer service.

The Bad

Protocol optimisation required.

The Bottom Line

Good antibody, stains well.

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