DuoSet Mouse IFN-gamma

University of Montana
DBS
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

R&D Systems

Product Name:

DuoSet Mouse IFN-gamma

Catalog Number:

DY485

In vivo experiments using transgenic mice were performed to establish the role of a specific transcription factor during a systemic immune response. ELISA of re-stimulated cultures were performed to assess T cell responses in an antigen-specific manner.

Experimental Design and Results Summary

Application

ELISA

Starting Material

Cell Culture Supernatant

Protocol Overview

Supernatants were stored at -20 and thawed for use in this kit. Manufacturer recommend protocols were performed with overnight incubations performed for coating plates with capture antibody.

Tips

Had to dilute samples as precipitate formed after addition of substrate.

Results Summary

The first assay wasn't readable as I strong precipitate formation. I diluted my samples to fix this issue.

Additional Notes

None

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Summary

The Good

Easy and straight forward

The Bad

Small complaint: the boxes that these come in have an insert to hold the reagents, but the reagents fall through every time so it's hard to keep them organized. A small hassle.

The Bottom Line

Great kit overall with clean results and straight forward protocol that requires minimal optimization.

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