MLLT1 Antibody Review Abnova

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abnova

Product Name:

MLLT1 monoclonal antibody (M01), clone 3H2

Catalog Number:

H00004298-M01

Our lab generates a lot of protein knockouts in mouse embryonic stem cells using crispr-cas9 technology. We use Western Blots and subsequent sequencing to screen potential knockout clones.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

This antibody works well. Strong clear signal in control wildtype well and faint signal in knockout sample wells.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong clear band at right size

The Bad

Faint nonspecific band below right band.

The Bottom Line

Pretty good antibody, would use again.

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