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Brilliant Violet 785™ Mouse IgG2a, κ Isotype Ctrl Antibody
400274
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Human synovial cells were stained with Brilliant Violet 785™ Mouse IgG2a, κ Isotype Ctrl Antibody to assess non-specific binding/Fc receptor binding.
Flow Cytometry
Synovial cells yielded by enzymatic digest
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.
NA
BD LSR X-20 Fortessa. Analysis using FlowJo v10
Antibody worked well to control for non-specific/Fc receptor binding and on difficult cells. Little/no background was visible.
None
Antibody worked well on difficult cells with little background.
No particular negative
Very good isotype control antibody