Cell Signaling Technology
IKKα (3G12) Mouse mAb
11930
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Growth arrested human cell line was treated with growth factor in time dependent manner and tested for its effect on IKKα steady state levels using its specific antibody in immunoblotting technique. Cell signaling antibody was selected because it can detect endogenous level of IKKα proteins in non-transfected cells
Western Blot
Human whole cell lysate
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscence detection in X-Ray film
In the present study, antibody used from Cell Signaling Technology for immudetection and has showed excellent specificity towards IKKα. Growth factor treatment did not have a effect on IKKα steady state levels.
Detected highly specific band of IKKα
Excellent antibody