R&D Systems
Human IL-33 Antibody
AF3625
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The aim of the study was to test the role of specific growth factor on IL-33 expression in human smooth muscle cell. Proteins from each treatment were extracted and resolved in SDS PAGE. Resolved proteins were western transferred in nitrocellulose membrane and immunoblotted with IL-33 antibody.
Western Blot
Human smooth muscle cell extract
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:5000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Excellent bright band of IL-33 was observed at around 30 kDa. There was no non-specific band observed with this antibody. However, there was positive time dependent effect of growth factor on IL-33 expression.
Gives very specific band
Excellent antibody