Abcam
Anti-JunB antibody [EPR6518]
ab128878
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Human cells were transfected with specific siRNA to test the role of siRNA target protein on growth factor induced JunB expression levels. After target protein knockdown human cells were subjected to growth arrest and treated with growth factor. Proteins extracts were made, resolved, and detected for JunB levels using its specific antibody. Abcam antibody flyer showed good detection of target protein so it was selected.
Western Blot
Human whole cell protein lysate
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:6000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
ECL detection in X-Ray film
Antibody from Abcam has detected JunB band at nearly 38 kDa. The band was bright and sharp. However, a non-specific bright band at 72 kDa was also observed.
Detected highly specific band of JunB at nearly 38 kDa
Best antibody for JunB detection