Santa Cruz Biotechnology Inc
Gα13 (6F6-B5)
sc-293424
Info: View Product View Product Specs
Browse Similar Products: Galpha13 Western Blot Antibodies
The aim of the study was to test the time course effect of growth factor on Gα13 interaction (association/disassociation) with growth factor specific G-protein coupled receptor (GPCR) in mammalian smooth muscle cells. Cells were time dependently treated with growth factor, proteins were extracted, equal amount of proteins were co- immunoprecipitated with receptor antibody. Co-immunoprecipitated cell lysates were incubated with specific protein A/G beads, immunocomplexes were resolved in 10% SDS-PAGE, and immunoblotted with anti- Gα13. Selection of antibody was based on compatibility of antibodies for immunoprecipitation information as provided in antibody flyer.
Western Blot
Whole cell lysate
Dilution (1:500) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:5000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Gα13 antibody from SantaCruz biotech has detected a specific band of Gα13 at nearly molecular weight of 40 kDa. However, time dependent disassociation of Gα13 from growth factor receptor was observed.
Detected Gα13 in immunoprecipitated samples
Nice antibody for Immunoblotting