Santa Cruz Biotechnology Inc
p-IκB-α Antibody
sc-8404
Info: View Product View Product Specs
Browse Similar Products: p-lkappaB-alpha Western Blot Antibodies
Mouse pancreatic endothelial cell line was tested for time course effect of specific growth factor on IκB-α phosphorylation. Cells which were grown in Medium 131 containing microvascular growth supplements (MVGS) were growth arrested, treated with growth factor for different time periods, proteins were extracted and equal amounts of proteins were resolved in 10%SDS-PAGE followed by immunoblotting with p-IκB-α specific antibody obtained from Santa Cruz Biotech.
Western Blot
Mouse pancreatic endothelial cell whole cell lysate
Dilution (1:500) in 5% BSA prepared in TBST, overnight incubation at 4 degree Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 2hr at RT
NA
ECL detection in X-Ray film
Although, anti- p-IκB-α from SantaCruz biotech has detected a specific band of p-IκB-α at nearly 35 kDa (indicated low range protein marker, Bio-Rad# 161-0304), some nonspecific bands were also observed. Intensity of p-IκB-α band was fairly weak but time dependent increase in phosphorylation was detected.
Weak detection of p-IκB-α band
Detected p-IκB-α
Antibody also cross-reacts with other nonspecific epitopes
Average antibody for detection