BioLegend
APC Mouse IgG1, κ Isotype Ctrl
400120
Info: View Product View Product Specs
Browse Similar Products: IgG1 Flow Cytometry Antibodies
Isotype control used in combination with Biolegend product # 313206 to account for non-specific in human synovial cells yielded by enzymatic digestion.
Flow Cytometry
Human synovial cells
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 5ug/ml.
NA
BD LSR X-20 Fortessa. Analysis using FlowJo v10
Antibody worked well detecting a low level of non-specific binding for this isotype in human synovial cell digests. When used in combination with Biolegend product #313206 CD117 cells were clearly identified.
None
Antibody performed as expected allowing quantification of non-specific binding
Antibody performed as expected of an isotype control