Pharmingen
Purified Mouse Anti-Human CD147
555961
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Browse Similar Products: CD147 Immunofluorescence Antibodies
CD147 is a marker that is associated with invasion and metastasis in a variety of malignant diseases. For our study, we needed to optimize CD147 immunostaining on Y79 human retinoblastoma cells. We chose this mouse monoclonal antibody, since it would pair well with a rabbit primary antibody for double immunostaining.
Immunofluorescence
Cytospins of Y79 human retinoblastoma cells
5.0 ug/ml in PBS with 0.05% Tween20, Room Temperature for one hour
No blocking agent
Vector Dylight 549 anti-mouse antibody (D12549) used at 5.0 ug/ml, Room Temperature for 45 minutes
Vectashield mounting medium containing DAPI counterstain
Fluorescence microscopy- Nikon microscope with SPOT camera
In the attached image, you can see very clear fluorescent staining of CD147 on the surface of the Y79 human retinoblastoma cells (red) in conjunction with the DAPI counterstain (blue). The level of background is very low.
None
Very clear staining, easy to optimize
Overall, this antibody was very easy to optimize, with the best results at 5 ug/ml. I recommend this antibody for use in fluorescent immunocytochemistry.