LSBio
Anti-SREBP-1c antibody
LS-B93
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This antibody was used in a study that investigated the role of hepatic PPARγ signaling in alcohol-induced liver injury. It has been published in Alcohol Clin Exp Res in 2016 (Zhang et al., Hepatic Peroxisome Proliferator-Activated Receptor Gamma Signaling Contributes to Alcohol-Induced Hepatic Steatosis and Inflammation in Mice. Alcohol Clin Exp Res. 2016 May;40(5):988-99). Anti-SREBP-1c antibody (cat. # LS-B93) was used for WB. This antibody gave very good results.
Western Blot
Mice liver
1:1000, 4 degrees, overnight
5% non-fat milk
1:1000, 1 hour, room temperature
NA
Supersignal west dura extended duration substrate
Western blot results showed that 8-week alcohol feeding significantly induced both precursor SREBP-1c and cleaved SREBP-1c. The activation of SREBP-1c was abolished by hepatic PPARγ knockdown.
None
Detection of specific bands for both precursor and cleaved SREBP-1c
Good antibody for the detection of both precursor and cleaved SREBP-1c in Western-Blot.