PE-Conjugated Monoclonal Mouse Anti-Human CD56 Antibody (for Flow Cytometry) from Miltenyi Biotec (Clone AF12-7H3)

May 24, 2016

Division of BioMedical Sciences, Faculty of Medicine
Memorial University of Newfoundland
Research Assistant

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Company:

Miltenyi Biotec

Product Name:

CD56-PE, human (clone AF12-7H3)

Catalog Number:

130-090-755

CD56 is expressed on the vast majority of resting and activated natural killer (NK) cells, as well as on a minor subset of CD3+ T cells. Our lab routinely uses this antibody to identify NK cells in peripheral blood samples. The product gives excellent results, making it very easy to distinguish between CD56+bright and CD56+dim populations.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human peripheral blood mononuclear cells isolated from whole blood by standard Ficoll density gradient separation

Primary Incubation

PE-conjugated anti-human CD56 antibody (Miltenyi 130-090-755), incubated with approximately 1x10^6 cells for 1 hour on ice at 1:10 dilution in a total volume of 100ul PBS+2% FCS, 1mM EDTA, 0.1% sodium azide.

Blocking Agent

NA

Secondary Incubation

NA

Tertiary Incubation

NA

Detection

FACSCalibur (Becton Dickinson)

Results Summary

This antibody worked excellent in FACS analysis of human PBMC. As expected, two populations of CD56-expressing cells were identified, one with high expression (bright) and another with lower expression (dim). Very little background staining compared to unlabelled cells was observed.

Additional Notes

For flow cytometric analysis, gating was performed on the lymphocyte population. Monocytes were negative (not shown).

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Summary

The Good

Bright staining. Little background. Comes ready to use with detailed staining procedures.

The Bad

None

The Bottom Line

This is an excellent product for staining of cells for flow cytometry. You should seriously consider this antibody if your area of research involves NK cell biology. Highly recommended!

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