BioLegend
PE anti-mouse/rat/human Foxp3 antibody
320008
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The general aim was to determine how T cells are differentiated following different stimulation conditions. This product was purchased because BioLegend is a company that I trust.
Flow Cytometry
Mouse T cells
Followed manufacturer's dilution. Was incubated in eBioscience permeabilization buffer.
NA
Fortessa
For this experiment, I fixed and perm my cells. For compensation, I used eBioscience's beads. This antibody was a poor antibody to detect Foxp3. When I was acquiring my experimental sample, most of the events was not showing within the dot plot and had to recompensate and alter the PMT voltage. A few scattered events were displaying in the Foxp3 positive gate, however I question the validity of the results.
None
Can be optimized
Bad fluorochrome
I would choose a different fluorochrome that is not PE.