Cell Signaling Technology
P-S6(240/244)
5364S
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This antibody was used to check endogenous levels of ribosomal protein S6 only when phosphorylated at Ser240 and Ser244.
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA
1:10000 dilution of anti-rabbit secondary antibody, 1hour at room temperature
NA
ECL
The band is very clear, and it has expression in the normal mice liver, and it increased in the liver cancer samples
None
A good antibody
The dilution of primary and secondary antibody could be adjusted based on the results, since it's a mono antibody, the results are very nice and repeatable.