Cell Signaling Technology
mTOR
2983S
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Browse Similar Products: mTOR Western Blot Antibodies
This antibody is used to detect endogenous levels of total mTOR protein at the dilution ratio of 1:1000 in 5% BSA or non-fat milk.
Western Blot
Tissue lysis or cell lines lysis
1:500 dilution, incubation overnight at 4 degrees
5% BSA or 5% non-fat milk
Goat anti-rabbit antibody
NA
ECL
There is not much differences between normal and tumor tissues, the band is at 289 kda size, the background could be reduced through changing the antibody dilution and ECL substrate exposure time.
None
A good antibody