Life Technologies
Goat Anti Mouse - HRP
A16072
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We use this product as a secondary antibody to ELISA and WB, using a primary antibody mouse anti-HIS. We choose this company and this product because Life Technologies is a very established supplier.
ELISA
Recombinant proteins with HIS-TAG
30uL bacterial extract with recombinant proteins
Caseín 1% in PBS
Anti-his tag 1:2000; overnight
Anti-mouse HRP 1:2000 1H RT
200uL TMB solution (from Life Technologies)
In this experiment, we found that the anti-mouse HRP works very well at this concentration. It was able to recognize our recombinant protein.
None
It is very stable at 4ºC
It is not easy to reconstitute
This antibody is very good and has a good price. Despite the difficulty to reconstitute, it is very easy to use.