Santa Cruz Biotechnology
GAPDH
SC-25778
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This antibody was used at 1:1000 dilution in 5% BSA incubation overnight, it's a poly-antibody and should stored at 4 degrees.
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA or 5% non-fat milk
Goat anti rabbit antibody
NA
ECL
It's a strong and clear band.
None
This is a good antibody
Though sometimes there were fewer unspecific bands, the results can be adjusted through changing the dilution of the primary or secondary antibody.