Cell Signaling Technology
Total Akt
4685S
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Browse Similar Products: Akt Western Blot Antibodies
We usually use 1:1000 dilution for total akt expression level in tissue or cell lines. This secondary antibody dilution can be used from 1:5000 to 1:10000..
Western Blot
Tissue lysis or cell lines lysis
1:1000 dilution, incubation overnight at 4 degrees
5% BSA or 5% non-fat milk
1:5000-1:10000 dilution , 1hour at room temperature
None
ECL
The band is very even and without unspecific bands.
A good antibody.
No specific requirement on it