EMD Millipore
Goat Anti-Rabbit IgG, HRP-conjugate
12-348
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The dilution used for this is 1:500 or 1:400, it is diluted in PBS and incubated for 30 minutes to 1 hour. This is a very reliable secondary antibody for checking protein expression level when using primary rabbit antibody.
Immunohistochemistry
Paraffin block slides
Primary antibody from rabbit incubation for 1 hour
Super block
Goat anti-rabbit antibody
NA
DBA
It's a very reliable secondary antibody for checking protein expression level when using primary rabbit antibody.
None
Good antibody
If detecting a low expression protein in the tissue, the dilution could decrease to 1:200. Usually use a dilution of 1:400.