Fast Semi-Dry Machine for Western Blot

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Company:

Bio-Rad

Product Name:

Trans-Blot Turbo

Catalog Number:

1704155

This semi-dry transfer machine can be used for western blot transfer in 7 minutes, it's faster than traditional wet transfer method, and the lab operator can adjust time based on the target protein size.

Experimental Design and Results Summary

Application

Western blot

Starting Material

Transfer buffer, western blot lysis samples, membrane

Protocol Overview

After the gel is finished running, take out the sandwich sponge and membrane and place in the 1x transfer buffer for 3-7 minutes before beginning to put them into the machine cassette, and then put the sandwich on the cassette, put the membrane on the sponge, take the gel to the membrane, use the stroll to pull out the bubble, and put the secondary sponge on the top. Close the box and then set up transfer program based on protein size.

Tips

Putting the sandwich sponge and membrane into transfer buffer longer may be better, transfer time can be increased based on protein size

Results Summary

It can be used for proteins from 10-300 kda, with mix molecular transfer program.

Features Summary

It's fast, reliable, stable, and repeatable

Additional Notes

None

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