Time Course Effect of Chemoattractant on ABCA1 Expression

May 18, 2016

Internal Medicine
Texas Tech University Health Science Center
Postdoctoral Researcher

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Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

ABCA1

Catalog Number:

ab18180

ATP-binding cassette transporter is a transporter which regulates the cellular cholesterol and phospholipid homeostasis. Here we checked the time course effect of ABCA1 by chemoattractant.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse cell lysate

Primary Incubation

1:1000 in 5%Milk prepared in 1xTBST

Blocking Agent

5% Milk prepared in 1x TBST

Secondary Incubation

1:4000 5%Milk prepared in 1XTBST

Tertiary Incubation

NA

Detection

ECL

Results Summary

We found a time dependent increase of ABCA1 by chemoattractant.

Additional Notes

It is high molecular weight protein. Use 7.5% gel to detect the band prominently.

Related Categories

Image Gallery

Summary

The Good

The antibody works effeciently and the results are accurate and easy to optmize.

The Bad

None

The Bottom Line

ABCA1 is a important transporter that transports the cellular mediates efflux of cholesterol and phospholipids to lipid poor apo-A1 and apoE. This antibody works efficiently to detect this protein. Highly recommended.

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