Sigma-Aldrich
Trypsin Profile IGD Kit
PP0100
The enzyme Trypsin is found in the digestive system where it hydrolyses proteins. Trypsin cleaves peptide chains mainly at the carboxyl side of the amino acids lysine or arginine, except when either is followed by proline. After protein purification, it has to be convert to peptides and these peptides are used further the mass spectrometers analysis.
To identify the mass of protein in the sample
Obesity protein samples
After completed the 2D gel run, pickup the interested protein bands and follow the steps as per the manufacturer’s protocol.
Cut the total area of the interested bands.
I observed good results with this kit
None
Good results with simple steps
Expensive and does not have long life.
Useful for labs where protein structural studies are a concern.