Compact Basic Sonicator for Cell Disruption

Protocol Overview

The included 3.2 mm tip is rated for 0.5 mL to 15 mL. The optional 2 mm tip is rated for 0.2 mL to 5 mL. The optional tip works better for the 1-2 mL volumes that are typical for our lab. The sample is placed on ice or cold rack and sonicated in pulses to avoid overheating. Note that the photo shows a student feeling out the tip depth before sonication. It can be tricky to get the right depth when the tip is obscured by the rack.